#FTIR DATA WITH ESSENTIAL FTIR HOW TO#

We finally demonstrates the elemental analysis is orthogonal to all FTIR markers as no significant correlation is found between FTIR and LA-ICP-MS data.

#FTIR DATA WITH ESSENTIAL FTIR HOW TO#

We show how to bring the images obtained by the two methods to a same spatial resolution and how to use image registration to analyze the data originating from both techniques as one block of data. The results bring the proof of concept that breast tissue can be analyzed simultaneously by FTIR spectroscopy and laser ablation elemental analysis (LA-ICP-MS) to provide in both case reasonably high resolution images. The 6 tissue sections provided 31 10 6 and 150,000 spectra for FTIR and LA-ICP-MS spectra respectively. Fourier transform infrared (FTIR) spectroscopy images reported a fingerprint of the organic molecules present in the tissue section and laser ablation elemental analysis (LA-ICP-MS) images brought inorganic element profiles. For illustrating the method, 6 mammary tissue sections were used. The purpose of this work is to demonstrate for the first time that elemental analysis and Fourier transform infrared spectroscopy microscopic examination of breast tissue sections can be merged into one dataset to provide a single set of markers based on both organic molecules and inorganic trace elements.

Yet, these approaches do not usually provide a sufficiently detailed characterization of the pathology. Histopathology and immunohistology remain the gold standard for breast cancer diagnostic.

Ftir data with essential ftir